Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Article Title: A Milk Extracellular Vesicle-Based Nanoplatform Enhances Combination Therapy Against Multidrug-Resistant Bacterial Infections.
doi: 10.1002/advs.202406496
Figure Lengend Snippet: Figure 6. Improved of the therapeutic effect in a peritonitis–sepsis infection model in mice by using FMEV. A) Scheme of the experimental protocol for the mouse peritonitis–sepsis model. B) Survival rates of mice with peritonitis–sepsis (n = 7). Mice were infected with a lethal dose of E. coli B2 (4.0 × 107 CFU) in the presence of PBS, PB (8 mg kg−1), PLU (16 mg kg−1), PB + PLU (8 mg kg−1 + 16 mg kg−1), and FMEV (PB: 8 mg kg−1 + PLU: 16 mg kg−1). P-values were determined using the two-sided, log[rank] (Mantel–Cox) test. *p < 0.05. C–G) Bacterial load (expressed as Log10 CFU) of E. coli B2 present in the heart (C), liver (D), spleen (E), lung (F), and kidney (G); this was evaluated at 48 h via a tenfold serial dilution technique. H) Representative photomicrographs of the H&E and Masson staining of the heart, liver, spleen, lung, and kidney. The arrow indicates the pathological lesions. The results are presented as the mean ± s.d. (n = 7). Significance was determined by Tukey’s multiple comparisons test following one-way ANOVA. ns, not significant. **p < 0.01 and ***p < 0.001.
Article Snippet: CAMHB broth (HB6231-1), BHI broth (HB8297-1), agar powder (HB8274-1), Salmonella chromogenic medium (HB7007-1), E. coli chromogenic medium (HB7003-7) were purchased from Haibo (Qingdao, China).
Techniques: Infection, Serial Dilution, Staining